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My lists My alerts. Please select your preferred region. Twitter icon Facebook icon Pinterest icon. Add a alert Enter prices below and click 'Add'. Alert if:. New Price below. A major disadvantage of EST-derived microsatellites is the sequence redundancy that yields multiple set of markers at the same locus.
Application of microsatellites in plant breeding. Microsatellites have been used for plant genetic analysis such as to measure the effects of natural selection Rodrigues and Santos , to unveil the genetic diversity Vieira et al.
Marker-assisted plant breeding involves the application of molecular marker techniques and statistical and bioinformatics tools to achieve plant breeding. The book 'Marker-Assisted Plant Breeding, Principles to Practice' is designed for such students who have had little or no exposure to molecular markers, but.
Using microsatellite in genetic diversity and population structure in common bean. Molecular marker analysis has contributed for the understanding of common bean genetic structure diversity and phylogenetic analysis Asfaw et al. For molecular markers, such as RAPD, SSR, and RFLP, there were major findings such as plant population continuous variation and high level of dissimilarity within population and among germplasm collections. Microsatellite markers are involved not only in genetic diversity studies, population genetics and evolutionary studies, but are also being used in fundamental research like genome analysis, gene mapping and marker-assisted selection Kalia et al.
Genetic divergence and population structure in common bean using microsatellite. The assessment of genetic diversity and population structure of accessions of common bean from Brazil and Africa was performed using microsatellites, genetic distance estimates and probabilistic models to analyze population structure.
Sixteen microsatellite markers from all the 11 linkage groups of P. Markers originated from genic and genomic sequences were chosen in equal proportions Yu et al. Microsatellite analysis was conducted as described by Kwak and Gepts , including an economic method of microsatellite fluorescent labeling of fragments amplified by PCR Schuelke Microsatellite methods employing fluorescent labeling and automated band calling with precise software-based allele detection are considered the most accurate way of genotyping McCouch et al.
Additionally, this sort of labeling organizes markers into distinct dye color panels allowing multiplexing during band separation, with advantages of high throughput genotyping and simultaneous analysis of multiple loci Coburn et al. The methodology of multiplexing can also be carried out in PCR amplification step with mixtures of the appropriate primers Hayden et al. Current technology allows capillary-based separation of microsatellite bands with four different color panels, one for each individual marker, which can be evaluated through a single capillary with a separate size standard, allowing precise band size estimates Coburn et al.
The amplified fragments were multiplexed depending on their size variation and analyzed in an ABI Applied Biosystems. Genotypes of markers were determined using the GeneMarker program version1. The genetic relationship among entire accessions was analyzed by principal coordinate analysis performed using the GenAlex 6 Program Peakall and Smouse The genetic distance among Andean and Mesoamerican accessions was calculated using the C. Chord distance Cavalli-Sforza and Edwards The C. Chord distance does not require any mutation model for microsatellite evolution and is free from bottleneck effect Takezaki and Nei Based on this genetic distance, an unrooted neighbor-joining NJ tree was constructed in PowerMarker Liu and Muse Distinct clusters were apparent with the SSR markers unambiguously assigning accessions to the Andean and Mesoamerican gene pools with the 2D plot of the PCoA based on pairwise genetic distances.
The obtained results demonstrated that Andean gene pool accessions from Brazil and Africa were clustered together showing a great genetic similarity. Likewise, the Mesoamerican accessions from both countries formed a second group.
These results indicate the possibility of introgression between countries. Some African accessions with low genetic similarity could be use in breeding programs to broaden the genetic base of Brazilian cultivars. Potential application of SSR markers for mapping and tagging disease resistance genes common bean. Crous e U. Braun, are the most widespread, recurrent and devastating diseases of common bean in Latin America and Africa. Use of disease resistance genes is the most practical, cost-effective and environmentally friendly strategy for the control of ANT and ALS.
Resistance to various diseases in common bean is conferred mostly by single, dominant genes with race-specific resistance R-genes. Resistance to C. Six independent dominant genes identified by the Phg symbol condition resistance to P. A total of individual F 2 derived from a cross between resistant and susceptible parents were inoculated with races 73 of C.
The analysis was performed using 22 SSR markers. Two contrasting DNA bulks Michelmore et al.
Of these 22 SSR markers, the g showed contrasting amplification patterns in parental materials and in resistant vs. The results of molecular mapping revealed that the SSR marker g was linked at 0. The co-segregation between these genes revealed the possibility of monitoring those genes indirectly through the use of g molecular marker.
In this study, the findings might help plant breeding programs to reduce time and cost associated with pyramiding resistance genes to different pathogens. Molecular Breeding 27 : Allard RW Principles of plant breeding. Theoretical and Applied Genetics : Genetics and Molecular Biology 28 : Genetic Resources and Crop Evolution 54 : Nucleic Acids Research 27 : Fruits 54 : Crop Breeding and Applied Biotechnology 5 : American Journal of Human Genetics 19 : Hereditas : Charcosset A and Moreau L Use of molecular markers for the development of new cultivars and the evaluation of genetic diversity.
Euphytica : Crop Science 42 : Indian Journal of Genetics and Plant Breeding 44 : Accurancy assessment. Genome Research 8 : Crop Science 51 : Molecular Genetics and Genomics : Genetic and Molecular Resource 6 : Genetic Resources and Crop Evolution 57 : Genome 50 : Molecular Breeding 21 : HortScience 39 : Biotechniques 16 : Kwak M and Gepts P Structure of genetic diversity in the two major gene pools of common bean Phaseolus vulgaris L.
Constructing genetic maps with Mapmaker Exp 3. Whitehead Institute for Biometrical Research, Cambridge, p.